MECHANISMS OF QUANTUM AND MULTIVESICULAR RELEASE OF NEUROTRANSMITTERS AND THEIR RELATIONSHIP TO ENDOCYTOSOSIS IN CENTRAL NERVOUS SYSTEM SYNAPSES

Operation co-funded by EU cohesion policy (European Regional Development Fund (ERDF)) under the programme Multi-regional Spain - ERDF (2014ES16RFOP002), managing authority: Subdirección General de Gestión del FEDER, de la Dirección General de Fondos Europeos del Ministerio de Hacienda.. The source names no beneficiary for this operation. Location: Seville Province (NUTS ES618); Spain. Budget: total eligible expenditure EUR 145,200; EU co-financing EUR 116,160 (co-financing rate 80%), as stated by the source. Runs 2016-01-01 to 2019-09-30. Source: Kohesio, EU cohesion policy projects managed by national and regional authorities (not CORDIS); selected because its title uses a quantum word. Summary from Kohesio (English machine translation by the source): THE NORMAL FUNCTIONING OF THE BRAIN LIES IN THE PRECISE AND CONTROLLED FUNCTION OF SYNAPSES, THE ELEMENTS THAT CONNECT NEURONS TO EACH OTHER FOR THE TRANSMISSION OF INFORMATION. CONTRARY TO WHAT MIGHT SEEM, VERY RECENT EXPERIMENTS SHOW THAT THE CLASSIC MODEL OF SYNAPTIC FUNCTIONING OF NEUROTRANSMITTER RELEASE AND SYNAPTIC VESICLE RECYCLING MAY BE DIFFERENT FROM WHAT IS ESTABLISHED. FIRST, THE EXISTENCE OF MULTIVESICULAR RELEASE IN NEURONS OF HIPPOCAMPUS, CEREBRAL CORTEX, CEREBELLUM, AND OTHER AREAS OF THE CENTRAL NERVOUS SYSTEM (CNS) CONTRASTS WITH THE CLASSICAL QUANTIC HYPOTHESIS OF THE CASTLE AND KATZ. SECOND, THE EXTREME SPEED (MS) OF ENDOCYTOSIS IN AREAS VERY CLOSE TO THE RECENTLY DESCRIBED ACTIVE ZONE CONTRASTS WITH CLATRINE-MEDIATED ENDOCYTOSIS CLASSIC PATHWAY IN PERIPHERY AREAS OF SYNAPSES. Our GROUP has worked in the cycle of synaptic visions and in the multivesicular release DURANT YEARS, WORKS that have had an IMPORTANT repercussion, VARIOS OF THE MECANISMS FOR US PROPOSES, MEDIANCE FREEDOM KISS AND RUN OR THE DIRECT DEMOSTRATION OF QUANTICATION OF NEUROTRANSMISERS medium AMPEROMETRY IN CROMAFINE CELLS AND MASTOCITS FOR NEUROCIENCE TEXT BOOKS OR CELL BIOLOGY. in the present project will amplify the multivesicular release pad and the identity of the MEMBRANA reciclosed in proximous zones to the active zone by means of the use of electrophysiology techniques, application OF FLUORESCENCE FLUORESCENCE OF HYPOCAMPO NEURONS THAT EXPRESSING Genetically MODIFIED SONDES (sinaptophysine-fluorine, SYPHY) and ELECTRONIC MICROSCOPY. THE SPECIFIC OBJECTIVES OF THIS PROPOSAL ARE TO: 1) STUDY OF QUANTUM AND MULTIVESICULAR RELEASE IN HIPPOCAMPUS NEURONS IN CULTURE USING FLUORESCENCE TECHNIQUES. EXPERIMENTS ON TRANSFECTED NEURONS WITH SYPHY SHALL BE CARRIED OUT AND THE SECRETORY RESPONSE IN SINGLE BUTTONS SHALL BE QUANTIFIED BY IMAGE ANALYSIS; 2) DEPENDENCE ON CALCIUM EXTRACELLULAR CALCIUM CONCENTRATION OF MULTIVESICULAR RELEASE IN HIPPOCAMPUS NEURONS. THE SAME TYPE OF [shortened; the full text is on the Kohesio page]

Original source.

Source: Kohesio (EU cohesion policy projects), European Commission, Directorate-General for Regional and Urban Policy (DG REGIO). Retrieved 2026-10-21. Licence: CC0 1.0 (Cohesion Open Data Platform, dataset 2014-2020 Kohesio projects). Selected metadata, adapted presentation; the publisher's record is authoritative. No endorsement by the source is implied. Licence: public domain dedication (CC0 1.0).

AI-assisted summary: The project investigates the mechanisms of neurotransmitter release and endocytosis in central nervous system synapses, co-funded by the EU with a budget of EUR 145,200. It ran from January 1, 2016, to September 30, 2019, in Seville Province, Spain. Suggested technology pillar: sensing. Generated by AI from the public source text; it may contain errors and the original source is authoritative.